Chromomycin A3 (CAS 7059-24-7): Suppliers, Product Information, and FAQs

Chromomycin A3 is an anthraquinone antibiotic and antitumor agent isolated from Streptomyces griseus. It is used as a fluorescent probe for DNA, specifically binding to GC-rich regions. It exhibits antibacterial, antifungal, and antitumor activities, and can inhibit DNA synthesis by inhibiting DNA gyrase. Chromomycin A3 is cytotoxic against non-small cell lung cancer and cervical cancer in vitro, with IC50 values ranging from 1.42 to 60 nM for various cell lines.

Frequently Asked Questions about Chromomycin A3

Q: What is the primary use of Chromomycin A3?

A: Chromomycin A3 is primarily used as a fluorescent DNA stain in flow cytometry and karyotype ***ysis of chromosomes. It is also used as a G-C specific DNA ligand that inhibits transcription.

Q: How does Chromomycin A3 work?

A: Chromomycin A3 binds reversibly to GC-specific DNA in the minor groove, which inhibits transcription, DNA gyrase, and topoisomerase II activity. It also antagonizes enhanced DNA binding of transcription factors Sp1 and Sp3 induced by oxidative stress or DNA damage.

Q: What are the potential applications of Chromomycin A3 in research?

A: Chromomycin A3 has potential applications in cancer research due to its cytotoxic effects on certain cancer cell lines. It is also useful for characterizing heterochromatin in plants with species-specific AT/GC ratios and for detecting protamine deficiency in sperm chromatin.

Q: How should Chromomycin A3 be stored?

A: Chromomycin A3 should be stored at -20°C. Solutions in DMSO may be stored at -20°C for up to 1 month.