Demecolcine (CAS 477-30-5) Suppliers and Product Information

Demecolcine, also known as Colcemid, is a microtubule polymerization inhibitor used in various research applications, including cell synchronization, karyotyping, and embryonic cloning studies. It acts by disrupting spindle fiber formation and can induce apoptosis in certain cell lines.

Frequently Asked Questions

Q: What is the primary use of Demecolcine?

A: Demecolcine is primarily used as a cell synchronization agent for chromosome visualization and to induce oocyte enucleation for somatic cell cloning. It is also used in karyotyping and cell cycle research to increase the yield of mitotic cells in a particular phase of the cell cycle.

Q: How does Demecolcine work?

A: Demecolcine inhibits tubulin polymerization, suppressing microtubule dynamicity at low concentrations and blocking microtubule assembly at higher concentrations. This action arrests cells in metaphase, making it useful for synchronizing cells and studying cell division processes.

Q: What are the safety considerations when handling Demecolcine?

A: Demecolcine is toxic and should be handled with care. It is classified as Acute Toxicity Category 2 for oral exposure. Proper personal protective equipment should be used, including gloves, protective clothing, and eye protection. It is also air and light sensitive, requiring proper storage conditions.