7-Aminoactinomycin D (CAS 7240-37-1): Suppliers and Product Information

7-Aminoactinomycin D (7-AAD) is a red-fluorescent DNA stain that is membrane impermeant to live cells but can penetrate dead or damaged cells. It is commonly used for the detection of non-viable cells in flow cytometric ***ysis and for evaluating apoptosis. 7-AAD has excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm, making it suitable for use in conjunction with other fluorescent probes like FITC due to minimal spectral overlap.

Frequently Asked Questions

Q: What is the primary use of 7-Aminoactinomycin D?

A: 7-Aminoactinomycin D (7-AAD) is primarily used as a fluorescent DNA stain for apoptosis detection and flow cytometry studies. It is particularly useful for identifying non-viable cells in cell populations.

Q: How does 7-AAD work in cell viability assays?

A: 7-AAD is membrane impermeant to live cells but can penetrate dead or damaged cells. This property allows it to stain the DNA of non-viable cells, making it an effective tool for distinguishing between live and dead cells in flow cytometry and other cell-based assays.

Q: What are the spectral properties of 7-AAD?

A: 7-AAD has excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm. These spectral properties allow it to be used in combination with other fluorescent probes, such as FITC, with minimal spectral overlap.

Q: How should 7-AAD be stored?

A: 7-AAD should be stored at -20°C. It is stable for 1 year from the date of purchase when supplied and stored properly. Solutions should be made fresh daily for optimal performance.