Puromycin Dihydrochloride (CAS 58-58-2) Suppliers and Product Information

Property	Value
Product Name	Puromycin Dihydrochloride
CAS Number	58-58-2
Molecular Formula	C22H30ClN7O5
Molecular Weight	507.98
Purity	≥98% (varies by supplier)
Appearance	White to yellow-white powder
Solubility	Soluble in water (50mg/mL)
Storage	Store in freezer under -20°C, inert atmosphere

Puromycin Dihydrochloride is an aminonucleoside antibiotic derived from Streptomyces alboniger. It acts as a protein synthesis inhibitor by causing premature chain termination during translation. It is commonly used as a selection agent in cell culture and as a research tool for studying protein synthesis.

Key features:

- Inhibits protein synthesis by disrupting peptide transfer on ribosomes - Inhibitor of dipeptidyl peptidase II and cytosolic alanyl aminopeptidase - Used for selection and maintenance of cell lines expressing transfected pac gene - Greater water solubility compared to puromycin free base

Handling precautions:

- Heat sensitive - Incompatible with strong oxidizing agents - Sterilize stock solutions by filtration (0.22 μm filter) - Store aliquots at -20°C

Frequently Asked Questions

Q: What is the main use of Puromycin Dihydrochloride?

A: Puromycin Dihydrochloride is primarily used as a selection agent in cell culture experiments and as a research tool for studying protein synthesis. It is also used for the maintenance of cell lines expressing the transfected pac gene.

Q: How does Puromycin Dihydrochloride work?

A: Puromycin Dihydrochloride acts as a protein synthesis inhibitor by causing premature chain termination during translation. It interferes with peptide transfer on ribosomes, leading to the release of incomplete polypeptide chains.

Q: How should Puromycin Dihydrochloride be stored?

A: Puromycin Dihydrochloride should be stored in a freezer at -20°C under an inert atmosphere. Stock solutions should be sterilized by filtration using a 0.22 μm filter and stored in aliquots at -20°C.

Q: What is the solubility of Puromycin Dihydrochloride?

A: Puromycin Dihydrochloride is soluble in water, with a solubility of approximately 50mg/mL.

Company Name	Contact	Product Details
Career Henan Chemical Co	Tel: 86 0371 86658258, 8613203830695	Purity: 95%-99%, Package: 1kg
Chongqing Chemdad Co., Ltd.	Tel: 86 023 6139 8061, 86 86 13650506873	Purity: ≥98%, Package: 1kg, 2kg, 5kg, 10kg, 25kg
CONIER CHEM AND PHARMA LIMITED	Tel: 8618523575427	Purity: ≥99%, Package: 1kg
TargetMol Chemicals Inc.	Tel: 1 781 999 5354	Purity: 99.73%, Package: 10mg, 50mg, 100mg, 200mg
Hubei Ipure Biology Co., Ltd.	Tel: 8613367258412	Purity: ≥99%, Package: 1kg, 5kg