Kanamycin Sulfate (CAS 25389-94-0) Suppliers and Product Information

Kanamycin sulfate is a broad-spectrum aminoglycoside antibiotic produced by Streptomyces kanamyceticus. It is effective against gram-negative and gram-positive bacteria, as well as mycoplasma. The compound shows strong antibacterial activity with lower toxicity compared to earlier aminoglycosides like streptomycin and neomycin.

Uses: - Treatment of serious infections caused by sensitive bacteria - Selection agent in isolation media and cell culture applications - Treatment of tuberculosis, dysentery, and salmonellosis - Additive in culture media for isolation of group D streptococci - Selection of transformed plant cells containing neomycin phosphotransferase

Frequently Asked Questions about Kanamycin Sulfate

Q: What is the recommended concentration for using kanamycin sulfate in cell culture?

A: The recommended concentration for kanamycin sulfate in cell culture applications is typically 100 μg/mL.

Q: How should kanamycin sulfate be stored?

A: Kanamycin sulfate should be stored at 2-8°C and protected from moisture. Stock solutions can be stored at 2-8°C and remain stable at 37°C for up to 5 days.

Q: What is the mechanism of action for kanamycin sulfate?

A: Kanamycin sulfate acts by binding to the 70S ribosomal subunit, inhibiting translocation and causing miscoding during protein synthesis.

Q: How is bacterial resistance to kanamycin developed?

A: Bacterial resistance to kanamycin can develop through aminoglycoside-modifying enzymes, including acetyltransferase, phosphotransferase, and nucleotidyltransferase. These enzymes alter the antibiotic, preventing its interaction with ribosomes.