Thiolutin (CAS 87-11-6) Suppliers and Product Information

Thiolutin is an antibiotic isolated from Streptomyces albus strains. It is a natural dithiol that reversibly inhibits bacterial and yeast RNA polymerases. Thiolutin has been used in research to study transcription processes, mRNA stability, and cell adhesion. It also shows potential as an angiogenesis inhibitor and antineoplastic agent.

Key biological activities: - Inhibits bacterial and yeast RNA polymerases (IC50 ≈ 3 μg/ml) - Inhibits endothelial cell adhesion (IC50 ≈ 1 μM) - Suppresses tumor cell-induced angiogenesis - Inhibits mannan and glucan formation in fungi - Inhibits JAMM metalloproteases

Frequently Asked Questions about Thiolutin

Q: What is the main use of Thiolutin in research?

A: Thiolutin is primarily used as an inhibitor of RNA polymerases in bacterial and yeast cells. It is often employed to study mRNA stability, transcription processes during cell cycles, and the effects of transcription inhibition on various cellular functions.

Q: How does Thiolutin affect angiogenesis?

A: Thiolutin has been shown to inhibit endothelial cell adhesion and suppress tumor cell-induced angiogenesis. It does this by perturbing Hsp27 interactions with components of the actin and intermediate filament cytoskeleton, which reduces paxillin levels in human umbilical vein endothelial cells (HUVECs).

Q: What precautions should be taken when handling Thiolutin?

A: Thiolutin is toxic by ingestion and subcutaneous routes. When heated to decomposition, it emits very toxic fumes of NOx and SOx. Proper personal protective equipment should be used, and the compound should be handled in a well-ventilated area. Always refer to the material safety data sheet (MSDS) for comprehensive safety information.