Gramicidin A (CAS 11029-61-1) Suppliers and Product Information

Gramicidin A is a naturally-occurring ion channel-forming pentadecapeptide derived from Bacillus brevis. It is an effective antibiotic against Gram-positive organisms and interferes with cell membrane permeability. Gramicidin A causes K /H exchange in mitochondria and is classified as an ionophore antibiotic.

**Uses:** Gramicidin A is primarily used as an antibiotic against Gram-positive bacteria. It is also used in research for studying ion channels and membrane permeability.

**Purification:** Gramicidin A can be purified by countercurrent distribution from C6H6-CHCl3-MeOH-H2O (15:15:23:7) with 5000 tubes. Final purification involves recrystallization from EtOH-H2O and drying at 100°C at 10^-2 mm over KOH.

Frequently Asked Questions

**Q: What is the mechanism of action of Gramicidin A?**

A: Gramicidin A acts by forming ion channels in cell membranes, particularly affecting the permeability of monovalent cations. This disrupts the normal ion balance in bacterial cells, leading to their death.

**Q: Is Gramicidin A effective against Gram-negative bacteria?**

A: No, Gramicidin A is primarily effective against Gram-positive bacteria. It has limited activity against Gram-negative organisms.

**Q: How should Gramicidin A be stored?**

A: Gramicidin A should be stored at 2-8°C to maintain its stability.

**Q: Can Gramicidin A be used in combination with other antibiotics?**

A: While Gramicidin A is often used alone, it can be combined with other antibiotics in certain formulations. However, specific combinations should be determined by a healthcare professional or researcher based on the intended application.